Journal: Frontiers in Endocrinology

Article Title: Regulatory Actions of LH and Follicle-Stimulating Hormone on Breast Cancer Cells and Mammary Tumors in Rats

doi: 10.3389/fendo.2018.00239

Figure Lengend Snippet: Modulatory effects of gonadotropins on breast cancer (BC) cell expression/activation of follicular-stimulating hormone receptor (FSHR), LHR, moesin, and focal adhesion kinase (FAK). Protein extracts from (A) T-47D (estrogen-receptor positive) and (E) MDAMB-468 (estrogen-receptor negative) BC cells treated for 48 h with increasing concentration of LH and follicle-stimulating hormone (FSH) (5, 5 + 50, and 50 mUI/ml) were assayed using western blot analysis for their overall content of LHR, FSHR, moesin, p-Moesin T558 , FAK, p-FAKY 397 , and actin. (B–D,F–H ) LHR, FSHR, moesin, p-Moesin T558 , FAK, p-FAKY 397 densitometry values were adjusted to actin intensity, respectively, then normalized to the control sample. * P < 0.05 vs. corresponding control. The experiments were performed in triplicates and representative images are shown.

Article Snippet: Blocking was performed with PBS containing 3% BSA for 30 min. BC cells were incubated with antibodies vs. phospho-FAK Y397 and phospho-Moesin T558 (1:500, BD Transduction Laboratories) overnight at 4°C followed by incubation with a fluorescein-conjugated goat anti-rabbit/mouse secondary antibody (1:200; Vector Laboratories).

Techniques: Expressing, Activation Assay, Concentration Assay, Western Blot

Journal: Oncology Letters

Article Title: Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway

doi: 10.3892/ol.2018.8139

Figure Lengend Snippet: The expression of p-Src and p-Fak in the HL-60 cells with a high expression of DJ-1 in the nucleus induced by diallyl disulfide for 0, 5, 15, 30 or 60 min. (A) A representative western blot analysis presenting the expression of p-Src and p-Fak. (B) Comparisons between the expression levels of p-Src and p-Fak. *P<0.05 vs. the control. p-Src, phosphorylated Src protein; p-Fak, phosphorylated Fak protein; DJ-1, parkinsonism associated deglycase.

Article Snippet: Phosphorylated Src (p-Src) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA; cat. no. 2101; 1:1,000 dilution) and phosphorylated Fak (p-Fak) was purchased from Abgent, Inc. (cat no. AJ1285e; 1:1,000 dilution). β-actin monoclonal antibodies were purchased from Abgent, Inc. (cat. no. AM1021B-ev; 1:2,000 dilution).

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway

doi: 10.3892/ol.2018.8139

Figure Lengend Snippet: DADS affects the Src signaling pathway in a control group, empty vector group and high expression group. (A) Protein expression of p-Src, Src, P-Fak, Fak and integrin was examined using western blot analysis. (B) Quantified protein expression of p-Src and p-Fac across the three groups. *P<0.05 vs. the control. DADS, diallyl disulfide; DJ-1, parkinsonism associated deglycase; p-Src, phosphorylated Src protein; p-Fak, phosphorylated Fak protein.

Article Snippet: Phosphorylated Src (p-Src) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA; cat. no. 2101; 1:1,000 dilution) and phosphorylated Fak (p-Fak) was purchased from Abgent, Inc. (cat no. AJ1285e; 1:1,000 dilution). β-actin monoclonal antibodies were purchased from Abgent, Inc. (cat. no. AM1021B-ev; 1:2,000 dilution).

Techniques: Plasmid Preparation, Expressing, Western Blot

Journal: Oncology Letters

Article Title: Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway

doi: 10.3892/ol.2018.8139

Figure Lengend Snippet: DADS and Src inhibitor inhibit the Src signaling pathway in the control group, the empty vector group and the high expression group. Protein expression of p-Src, Src, P-Fak, Fak and integrin was examined by (A) western blot analysis and (B) quantitative analysis. *P<0.05 vs. the control. DADS, diallyl disulfide; p-Src, phosphorylated Src protein; p-Fak, phosphorylated Fak protein; DJ-1, parkinsonism associated deglycase.

Article Snippet: Phosphorylated Src (p-Src) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA; cat. no. 2101; 1:1,000 dilution) and phosphorylated Fak (p-Fak) was purchased from Abgent, Inc. (cat no. AJ1285e; 1:1,000 dilution). β-actin monoclonal antibodies were purchased from Abgent, Inc. (cat. no. AM1021B-ev; 1:2,000 dilution).

Techniques: Plasmid Preparation, Expressing, Western Blot